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Peripheral nervous system progenitors can be reprogrammed to produce myelinating oligodendrocytes and repair brain lesions.
Neural crest stem cells (NCSCs) give rise to the neurons and glia of the peripheral nervous system (PNS). NCSC-like cells can be isolated from multiple peripheral organs and maintained in neurosphere culture. Combining in vitro culture and …
Publication
Neural progenitors of the postnatal and adult mouse forebrain retain the ability to self-replicate, form neurospheres, and undergo multipotent differentiation in vivo.
Somatic stem cells are reservoirs to replace lost cells or damaged tissue. Cells with neural stem cell (NSC) characteristics can be isolated from the postnatal mammalian brain into adulthood and expanded as neurospheres. We addressed the ability of these …
Publication
Image-Guided Cardiac Cell Delivery Using High-Resolution Small-Animal Ultrasound
Open-chest cardiac injection is the preferred delivery method for cardiac gene and stem cell therapy in small animals, but it is invasive and the operator is unable to see the actual delivery site. High-resolution ultrasound has recently been developed …
Publication
Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography
The mouse is an important model for the development of therapeutic stem cell/bone marrow cell implantation to treat ischemic myocardium. However, its small heart size hampers accurate implantation into the left ventricular (LV) wall. Precise injections …
Publication
Injection of bone marrow cell extract into infarcted hearts results in functional improvement comparable to intact cell therapy.
We compared therapeutic benefits of intramyocardial injection of unfractionated bone marrow cells (BMCs) versus BMC extract as treatments for myocardial infarction (MI), using closed-chest ultrasound-guided injection at a clinically relevant time post-MI. …
Publication
Parthenogenetic stem cells for tissue- engineered heart repair
Uniparental parthenotes are considered an unwanted byproduct of in vitro fertilization. In utero parthenote development is severely compromised by defective organogenesis and in particular by defective cardiogenesis. Although developmentally compromised, …
Publication
Enhanced angiogenic and cardiomyocyte differentiation capacity of epigenetically reprogrammed mouse and human endothelial progenitor cells augments their efficacy for ischemic myocardial repair.
RATIONALE: Although bone marrow endothelial progenitor cell (EPC)-based therapies improve the symptoms in patients with ischemic heart disease, their limited plasticity and decreased function in patients with existing heart disease limit the full benefit …
Publication
Development and Validation of a Combined Photoacoustic Micro- Ultrasound System for In Vivo Oxygen Saturation Estimation
Photoacoustic (PA) Imaging can estimate the spatial distribution of oxygen saturation (sO2) and total hemoglobin concentration (HbT) in blood, and be co-registered with B-Mode ultrasound images of the surrounding anatomy. This study will focus on the …
Publication
Acoustic Angiography: A New Imaging Modality for Assessing Microvasculature Architecture
The purpose of this paper is to provide the biomedical imaging community with details of a new high resolution contrast imaging approach referred to as “acoustic angiography.” Through the use of dual-frequency ultrasound transducer technology, images …
Publication
Porphyrin shell microbubbles with intrinsic ultrasound and photoacoustic properties.
Porphyrin-phospholipid conjugates were used to create photonic microbubbles (MBs) having a porphyrin shell ("porshe"), and their acoustic and photoacoustic properties were investigated. The inclusion of porphyrin-lipid in the MB shell increased the yield, …