Study Tissue Ischemia and Blood Reperfusion in Real Time

Vasculature in the mouse brain, visualized with color Doppler during transient carotid artery occlusion.

3D rendered spectrally unmixed photoacoustic image of a stem-cell injected, ischemic (right) and control (left) mouse hindlimb. Oxyhemoglobin (red), deoxyhemoglobin (blue) and dye-labelled stem cells (green) are shown.

Plot of oxygen saturation (sO₂) over time in the left (pink) and right (orange) cortical and subcortical hemisphere ROIs  while restoring breathed oxygen to 100% from 7.5% post ischemia/hypoxia for stroke induction.  Ultrasound (top) and photoacoustic (bottom) coronal images in the right panel show a frame of mouse brain post ischemia/hypoxia.

Ultrasound (left) and photoacoustic (right) coronal images of a mouse brain post ischemia/hypoxia. The red, white and blue color represents a parametric map of oxygen saturation.  The right hemisphere does not fully recover sO₂ levels post-reperfusion/normoxia that are shown in the left hemisphere, indicating a cerebral infarct, especially in cortical and subcortical areas.

3D rendered power Doppler image of the mouse hindlimb showing blood flow. Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb, then relieved to allow reperfusion.

3D rendered photoacoustic image of the mouse hindlimb showing a parametric map of oxygen saturation with red denoting higher sO₂ values.  Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb, then relieved to allow reperfusion. Higher sO₂ is seen in the distal part of the limb post-reperfusion.

3D rendered photoacoustic image of the mouse hindlimb showing a parametric map of oxygen saturation with red denoting higher sO₂ values.  Application of a tourniquet made of tubing was used to restrict blood flow into the distal part of the limb. Much lower sO₂ is seen in the distal part of the limb during this acute ischemia.

3D rendered high-resolution ultrasound image of the mouse hindlimb showing a depression in the skin from a tourniquet made of tubing being used to restrict blood flow into the distal part of the limb.

3D rendered coronal ultrasound (greyscale) and photoacoustic (red) images of a mouse brain during ligation of the right common carotid artery. The red color represents higher oxygen saturation clearly showing lower sO₂ in the right hemisphere.