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Knockout of dihydrofolate reductase in mice induces hypertension and abdominal aortic aneurysm via mitochondrial dysfunction
Hypertension and abdominal aortic aneurysm (AAA) are severe cardiovascular diseases with incompletely de- fined molecular mechanisms. In the current study we generated dihydrofolate reductase (DHFR) knockout mice for the first time to examine its …
Publication
Increased Brain-Derived Neurotrophic Factor in Lumbar Dorsal Root Ganglia Contributes to the Enhanced Exercise Pressor Reflex in Heart Failure
An exaggerated exercise pressor reflex (EPR) is associated with excessive sympatho-excitation and exercise intolerance in the chronic heart failure (CHF) state. We hypothesized that brain-derived neurotrophic factor (BDNF) causes the exaggerated EPR via …
Publication
68 Ga-DOTA-E[c(RGDfK)] 2 Positron Emission Tomography Imaging of SHARPIN-Regulated Integrin Activity in Mice
Shank‐associated RH domain‐interacting protein (SHARPIN, alias SIPL1) is a cytosolic protein that plays a key role in activation of nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) and regulation of inflammation. Furthermore, SHARPIN …
Publication
GPR 30 reduces myocardial infarct area and fibrosis in female ovariectomized mice by activating the PI3K/AKT pathway
Aims—Estrogen plays an important role in cardioprotection. Animal experiments showed that the G-protein coupled estrogen receptor 30 (GPR30) specific agonist G1 could reduce post-ischemic dysfunction and inhibit cardiac fibroblast proliferation. However, …
Publication
Portal milieu and the interplay of multiple anti-diabetic effects after gastric bypass surgery
AIMS: Diabetes is a worldwide health problem. Roux-en-Y-Gastric-Bypass (RYGB) leads to rapid resolution of Type-2-Diabetes (T2D). Decreased hepatic insulin resistance is key but underlying mechanisms are poorly understood. We hypothesized that changes in …
Publication
Time Series Characteristics of Serum Branched-Chain Amino Acids for Early Diagnosis of Chronic Heart Failure
Chronic heart failure (CHF) is an ongoing clinical syndrome with cardiac dysfunction that can be traced to alterations in cardiac metabolism. The identification of metabolic biomarkers in easily accessible fluids to improve the early diagnosis of CHF has …
Publication
Ablation of Cardiac TIGAR Preserves Myocardial Energetics and Cardiac Function in the Pressure Overload Heart Failure Model
Despite the advances in medical therapy, the morbidity and mortality of heart failure (HF) remain unacceptably high. HF results from reduced metabolism-contraction coupling efficiency, so the modulation of cardiac metabolism may be an effective strategy …
Publication
Sex influences susceptibility to methamphetamine cardiomyopathy in mice
In this study, we created a mouse model of methamphetamine cardiomyopa- thy that reproduces the chronic, progressive dosing commonly encountered in addicted subjects. We gradually increased the quantity of metham- phetamine given to C57Bl/6 mice from 5 to …
Publication
Rapamycin attenuates a murine model of thoracic aortic aneurysm by downregulating the miR-126–3p mediated activation of MAPK/ERK signalling pathway
Thoracic aortic aneurysm (TAA) is fatal diseases, which leads to aortic rupture and sudden death. Blood pressure-lowering drugs are ineffective for most of the patients. Our previous study demonstrated the inhibition of endothelial secreted miR-126e3p by …
Publication
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. Human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), human cardiac fibroblasts (HCFs), …