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Use of Ultrasound with 3D Contrast to Assess Colorectal Cancer

This recent article by Freeling et al. highlights the benefits of using Ultra High Frequency (UHF) Ultrasound with 3D Non-linear contrast (NLC) to assess colorectal cancer in a murine model.

Article Summary:

  • Colorectal cancer (CRC) has a high recurrence rate and a 5 year survival of only 6-12%
  • In vivo animal models of CRC are ideal but are difficult to study longitudinally
  • Used Vevo ultrasound with 3D and non-linear contrast to perform 3D reconstruction of the mouse colon
  • Studied vascularity in normal and CRC mice in response to treatment with a vehicle or anti-cancer compound, longitudinally
  • To ameliorate imaging, gel was injected into the colon and atropine was administered to halt gut peristalsis
  • Able to determine volume of individual tumors as well as total tumor load
  • Able to determine vascular perfusion and total vascularity and relate this to tumor load
  • Results were correlated with histology at necropsy and found to be more precise than optical colonoscopy

Conclusion:

3D reconstruction of the mouse colon using ultra-high frequency ultrasound with non-linear contrast is a robust technique for studying murine models of CRC longitudinally. Use of targeted microbubbles should be considered in the future, allowing for more detailed preclinical studies.

Reference:

Freeling JL, Rezvani K. Assessment of murine colorectal cancer by micro-ultrasound using three dimensional reconstruction and non-linear contrast imaging. Mol Ther — Methods Clin Dev [Internet]. 2016 Nov 30;5(July):16070. Available from:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147881/

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