Image Guided Injections
Shripad shares: Tips and Tricks for Image Guided Injections
For myocardial or Embryo injections, 5ul is a sufficient volume. One inch 30 gauge needles are always good. These needles are not too rigid and flexible to inject through the body wall and skin. For embryonic injections pulled glass needles are used. Glass needles have a risk of breaking at the tip before injection into the embryos, as skin gets folded or stretched.
Downloading Software
Philippe Trochet shares instructions for Downloading Vevo Software
our Applications Specialist Philippe Trochet shares the instructions you need to download the latest Vevo Software from our website.
Creating annotations in VevoLab
Magdalena explains how to create annotations in VevoLab; annotations in cineloops can be a great tool for presentations or to keep track of important notes:
In VevoLAB you can use (1) the right-click button to open the dropdown menu.
On The Vevo2100 you can use the corresponding update button. Use either a customized annotation or create a generic text. (2)
Once an annotation is created you can use the right-click again to a.) Edit the text of the label or b.) Change label properties like: font style, color and size.
Apical 4 Chamber View and Mitral Valve Inflow
Kelly explains Apical 4 Chamber View and Mitral Valve Inflow:
Many researchers are beginning to look for other signs of cardiac dysfunction aside from traditional systolic functional measurements during rat or mouse echocardiography. With the increased prevalence of cardiac diseases associated with preserved systolic function (i.e. HFPEF), diastolic functional measurements are becoming increasingly important.
Copying the Standard Measurement Packages
Sandra shows how to sort measurements using copies of the standard measurement packages:
I like how all the measurements I do in one imaging series in VevoLab are summed up on the report page and that I automatically get an average value and standard deviation when I repeat measurements. But sometimes I need to keep the data separate within one imaging series because the measurements refer to multiple time points or different locations. Click to read more.
Split Screen
Sandra explains Split Screen:
Today I would like to introduce you to the split screen function, which is available on all current Vevo systems and in VevoLab. It is a great tool for longitudinal studies and for image optimization. I use split screen to display structures or organs in the transvers and sagittal view at the same time or to look at differences by comparing the live image to a previously acquired cine loop. Click to read more.
Left Lateral Decubitus Position
Kristiina explains Left Lateral Decubitus Position:
Although you would originally assume that echocardiography would be easier in rat than in mouse, anyone who has done both will quickly acknowledge that the former is actually more challenging. Despite the larger size of the rat heart, ultrasound scanning is more difficult due to increased skin thickness, increased lung artifact and the increased shifting of lung and heart within the thorax. These challenges are typically worsened for larger rats. Click to read more.
ECG signal on neonates and small rodents
Lindsey explains ECG signal on neonates and small rodents:
ECG signal on neonates and small rodents. Question: How do you obtain physiologial data from neonates and smaller rodent's whose arms cannot extend to the electrodes? Click to read more.