Pathogenesis of Renal Injury in the Megabladder Mouse: A Genetic Model of Congenital Obstructive Nephropathy
Susan E Ingraham, Monalee Saha, Ashley R Carpenter, Melissa Robinson, Ihab Ismail, Sunita Singh, David Hains, Michael L Robinson, Daniel a Hirselj, Stephen a Koff, Carlton M Bates, Kirk M McHughPediatric Research2010
Congenital obstructive nephropathy (CON) is the most common cause of chronic renal failure in children, often leading to end stage renal disease. The megabladder (mgb) mouse exhibits signs of urinary tract obstruction progressive renal failure following birth. This study examined the development of progressive renal injury in homozygous disease state of mgb−/− in utero mgb resulting in the development of hydroureteronephrosis and mice ( mgb−/− ). Renal ultrasound was utilized to stratify the mice, while surgical rescue was performed using vesicostomy. The progression of renal injury was characterized using a series of pathogenic markers including α- smooth muscle isoactin (α-SMA), TGF-β1, connective tissue growth factor (CTGF), E-cadherin, F4/80, Wilm’s Tumor 1 (WT-1), and paired box gene 2 (Pax2). This analysis indicated that − mice are born with pathologic changes in kidney development that progressively worsen in mgb−/ direct correlation with the severity of hydronephrosis. The initiation and pattern of fibrotic development observed in mgb−/− kidneys appeared distinctive from prior animal models of obstruction. These observations suggest that the mgb mouse represents a unique small animal model for the study of CON.