VevoCQ Software* allows advanced quantification of perfusion kinetics parameters with consistent, reliable and actionable data

VevoCQ™ is a powerful addition to nonlinear contrast imaging functionality on the Vevo^® 2100 imaging system. This software allows for the study of contrast uptake kinetics as well as late phase targeted enhancement. It provides advanced curve fitting algorithms for quantitative assessment of perfusion parameters as well as color-coded parametric images useful for qualitative assessment of the spatial distribution of the same parameters. The software is a post-processing tool which can be used on numerous tissues, organs and tumor models, including subcutaneous tumors, abdominal organs and hind limb muscles.

1. User-defined regions of interest selection (up to 4)
   The ability to define multiple areas of interest allows selection and detection of anatomical regions with differing perfusion kinetics to be chosen for investigation (i.e. tumor and surrounding normal tissue or multiple areas within a tumor).
   
   
   
   Figure 1 – Location of the four user defined regions of interest on the subcutaneous tumor
    
2. Curve fitting capabilities
   Three options (based on what protocol for microbubble administration is used) are available such that data is plotted and generated for both perfusion and targeted molecular imaging studies.
   
   
   
   Figure 2 – Bolus perfusion model curve fit algorithm as applied to each region of interest on the subcutaneous tumor model
    
3. Data quantification
   A multitude of quantification tools are available such that data and parameters can be generated that relate to perfusion kinetics as well as molecular imaging capabilities (this is derived from the curve fittings).
   
   Table 1 – Selection of VevoCQ bolus perfusion model parametric outputs 

   	Whole Tumor (green)	Bottom Right (yellow)	Upper Area (pink)	Bottom Left (turquoise)
   Peak Enhancement (a.u.)	29.61	8.21	41.73	8.32
   Time to peak (sec)	3.84	3.66	3.70	2.78
   Wash-in Rate (a.u.)	15.50	4.51	22.56	6.09

   Data is output in a TSV format (Excel).
   
   Table 2 – Output parameters for bolus perfusion model 

   PE	Peak Enhancement	[a.u.]
   AUC	Area Under the Curve, limited to peak opacification instant	[a.u.]
   RT	Rise Time	[s]
   TTP	Time To Peak	[s]
   WiR	Wash-in Rate (maximum slope)	[a.u.]
   PI	Perfusion Index (AUC / RT)	[a.u.]

    
   Table 3 – Output parameters for destruction-replenishment reperfusion model 

   rBV	relative Blood Volume (A)	[a.u.]
   mTT	mean Transit Time	[s]
   rBF	relative Blood Flow (rBV / mTT)	[a.u.]

    
4. Parametric imaging
   Allows a visualize display of the intensity of perfusion kinetics and molecular signal.
   
   
   
   
   
   
   
   Figure 3 – Parametric images from bolus perfusion model, (A) peak enhancement, (B) time to peak, (C) wash-in rate
    
   Images and videos can be exported in a variety of formats such as TIFF, Bitmap and WMV.

*  Copyright © Bracco Suisse SA - 2010.