Gene/Drug Delivery with low-frequency sonoporation

SoniGene™ System: Gene/Drug Delivery with low-frequency sonoporation

The SoniGene system provides an effective, reliable and efficient mechanism for the targeted delivery of genes or drugs at the cellular level.

The Vevo imaging systems are used to identify the region of interest and provide longitudinal assessment of the effect of the gene or drug delivered. Image-guided needle injection could also be used for regional delivery of the gene solution.

The SoniGene system delivers a low-frequency/high-powered ultrasound-pulse sequence and when used in conjunction with the microbubble contrast agents, will cause a sonoporation of the targeted cells and allow the gene or drug to transfect. Contrast agents also act as a carrier agent for the gene or drug to the targeted site.

Gene Delivery with microbubble contrast agents:

Gene mixed in suspension with microbubble contrast agent

Overview of Gene Delivery Workflow:

• Anesthetize and prepare animal for imaging
• Prepare contrast agent and gene for injection
• Mount the SoniGene probe in imaging station micro-injection system and line up the SoniGene probe with the Vevo image
• Inject gene and contrast agent solution into mouse
• Acquire Vevo image
• From the Vevo system initiate the low-frequency destruction sequence 

Vevo SoniGene Technical Specifications:

* ERA is Effective Radiating Area
** BNR is Beam Non-uniformity ratio
*** Half-Power Depth is calculated at 1MHz transmit using standard 0.3 db/cm/MHz