Quantify realtive perfusion and study biomarker expression
Microbubble contrast agents have been used in ultrasound imaging as a means of improving the visualization of blood flow with respect to the surrounding tissue. These micron-sized particles consist of a gas core surrounded by a lipid shell, and are injected intravenously into the circulatory system. Understanding the patterns of blood flow is a powerful tool for evaluating the differences between normal and pathological tissues.
There are two contrast imaging techniques, linear and nonlinear.
Linear contrast agent imaging refers to visualizing the microbubble contrast agents using B-Mode imaging and then applying a reference subtraction algorithm to apply a green contrast overlay on the image to aid in the visualization and quantification of the contrast specific signal. This type of imaging is available both on the Vevo 770 as well as on the Vevo 2100 system.
Nonlinear contrast agent imaging uses multiple ultrasound pulses in which the amplitude of the pulse is modulated, by performing this type of imaging the nonlinear response of the microbubbles to ultrasound pulses is utilized. The goal of this type of imaging is to suppress the tissue signal while increasing the detection of the contrast agents, providing a much more sensitive imaging technique. Nonlinear contrast imaging is only possible on the Vevo 2100 system using the MS200 and MS250 transducers.
Nonlinear contrast agent imaging in the mouse abdomen. Untargeted Vevo MicroMarker® Contrast Agents were injection IV through a tail vein cannulation,post processed green contrast overlay aids in the visualization of the contrast agent
Linear contrast agent imaging. Untargeted Vevo MicroMarker Contrast Agent bolus wash-in curve, showing flow in the tumor. The slope of the curve indicates relative blood velocity, while the plateau indicates relative blood volume.
Nonlinear contrast agent imaging in the mouse abdomen. Untargeted Vevo MicroMarker Contrast Agents were injection IV through a tail vein cannulation, and can be seen to perfuse through the kidney, pancreas, and spleen.
Nonlinear Contrast Agent Imaging.Untargeted Vevo MicroMarker Contrast Agent bolus wash-in curve, showing flow in the cortex and medulla of a mouse kidney. The slope of the curve indicates relative blood velocity, while the plateau indicates relative blood volume.
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