Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors.
Goessling W, North TE, Zon LI.
Stem Cell Program, Hematology/Oncology, Children's Hospital and Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts
Nat Methods. 2007 Jun 17; [Epub ahead of print]

• > Zebrafish are a valuable vertebrate model to study carcinogenesis, but noninvasive imaging is challenging because adult fish are not transparent. Here we show that tumors could be readily detected in vivo using high-resolution microscopic ultrasound (Vevo 770 micro-ultrasound, VisualSonics Inc.) in zebrafish. We successfully obtained tissue perfusion calculations and cellular aspirates, and analyzed tumor progression and response to treatment. Ultrasound biomicroscopy allows longitudinal studies of tumor development and real-time assessment of therapeutic effects in zebrafish.
  
  NOTE: This paper not only uses the Vevo 770 for 2D and 3D imaging and sizing of tumors in vivo in zebrafish, but MicroMarker contrast agents are also used in the protocols to successfully and quantitatively measure tumor vascularity and perfusion in these zebrafish models of liver cancer.